A novel approach to evaluate ELISA antibody coverage of host cell proteins—combining ELISA‐based immunocapture and mass spectrometry
نویسندگان
چکیده
منابع مشابه
ELISA reagent coverage evaluation by affinity purification tandem mass spectrometry
Host cell proteins (HCPs) must be adequately removed from recombinant therapeutics by downstream processing to ensure patient safety, product quality, and regulatory compliance. HCP process clearance is typically monitored by enzyme-linked immunosorbent assay (ELISA) using a polyclonal reagent. Recently, mass spectrometry (MS) has been used to identify specific HCP process impurities and monito...
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ژورنال
عنوان ژورنال: Biotechnology Progress
سال: 2020
ISSN: 8756-7938,1520-6033
DOI: 10.1002/btpr.2983